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Figure 9


Fig. 9. Effects of APC on RAS- or ß-catenin-induced proliferation in NIH 3T3 cells. (A) RAS-inducible NIH 3T3 cells were grown and transfected with 0.5 µg of either pCMV or pCMV-APC, and RAS was induced by doxcyclin treatment as described in Fig. 8A. The cells were fixed, permeabilized and incubated with BrdU antibody and subsequently with TRITC-conjugated goat anti-rabbit IgG. The relative intensities of TRITC-conjugated BrdU were measured using a FACS Vantage system (Becton-Dickinson Immunocytometry systems). The lower panel shows a quantitative analysis of the data in the upper panels. Each data point represents the average of three independent analyses. (B) RAS-inducible NIH 3T3 cells were grown and transfected with 0.5 µg of either pCMV or pCMV-APC together with or without 0.5 µg of pcDNA3.0 or pcDNA3.0-Flag-ß-catenin. Immunofluorescence staining and FACS analyses were performed and analyzed as described in A. The lower panel shows a quantitative analysis of the data in the upper panels. Each data point represents the average of three independent analyses.





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