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Figure 2


Fig. 2. Kazal1 is expressed in gland cells of intact H. vulgaris. (A-G) Kazal1-expressing cells were detected after whole-mount in situ hybridization with Fast-Red either in fluorescence (A-C,F,G, red) or in bright-fields conditions (A,C). To highlight the regions and the cells that do not express Kazal1, hydra were subsequently immunodetected with anti-{alpha}-tubulin (B,E, green). Arrows indicate the lower (A) and the upper (B) limits of the Kazal1 expression domain along the body column. In C, a closer view of the body column region shows that Kazal1-expressing cells are restricted to the endodermal layer. end, endoderm; ect, ectoderm; mes, mesoglea. (D-G) Confocal views of the endodermal layer. Kazal1-expressing cells contain secretory vacuoles (arrows) characteristic of gland cells. The star indicates the nucleus of a gland cell that does not express Kazal1. Bars, 150 µm (A,B), 40 µm (C), 16 µm (D-G).





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