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Fig. 3. The dsRNAi feeding strategy in hydra. (A) Scheme describing the production of dsRNAs in bacteria and the feeding of hydra with the bacteria-agarose mix. (B) Evolution of the size of the H. vulgaris populations exposed three times a week to unc-22 dsRNAs over 5 weeks; s.d. values correspond to four populations (50 hydra at t0) followed in two distinct experiments. (C) Variations in hydra body length over the first 2 weeks of unc-22 dsRNA treatment. The values correspond to the percentages of the animal lengths at each time point over their size at t0; s.d. values correspond to ten hydra. (D) Appearance of tentacle rudiments in head-regenerating halves exposed either 1x, 3x, 8x or 19x to unc-22 dsRNAs (15 hydra per condition).