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Figure 3


Fig. 3. FAK and CAS are in FRET proximity to PY-containing sites. (A) Single-color images, ratio images and FRET images are displayed for the indicated pairs. High FI values were detected for both dSH2-FAK and dSH2-CAS donor-acceptor pairs. Bar, 2 µm. (B) Quantification of FRET measurements with the indicated FRET pairs. The data are presented as mean ± s.e. of three experiments. (C) Immunoprecipitation of CAS from lysates of 293 cells expressing YFP-CAS together with CFP-dSH2 leads to the co-precipitation of CFP-dSH2 (left). CFP-dSH2 co-precipitates with FAK in immunoprecipitation experiments from lysates of 293 cells expressing YFP-FAK and CFP-dSH2 (right).





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