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Figure 4


Fig. 4. Paxillin, CAS, and FAK phosphorylation in focal complexes and focal adhesions. NIH 3T3 cells were fixed and labeled for paxillin (A,D,G); phosphospecific antibody to paxillin PY118 (B); phosphospecific antibody against the FAK autophosphorylation site PY397 (E); phosphospecific antibody directed against CAS PY165 (H). Merged images are shown in the right-hand column (C,F,I). Note that paxillin, FAK and CAS are phosphorylated on the specific PY sites in focal complexes and focal adhesions. Bars, 5 µm.





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