spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. The C-terminus of Sec15p physically interacts with Bem1p, whereas the N-terminus of Sec15p is required for the interaction with Sec10p. Cell growth, yeast lysate preparations, GST pull-down and western blotting procedures were as described in the Materials and Methods. (A) Schematic diagram of Sec15p constructs employed in this study. (B) Sec15-Bem1 complexes. Glutathione-Sepharose pull-down products from lysates of cells expressing GST-fusion products of Sec15 (Sec15 GST; NY2559) and Sec15-1 (sec15-1 GST; NY2560), and GST (NY2561), were analyzed by standard SDS-PAGE and western blotting with the indicated antibodies. (C) Glutathione-Sepharose pull-down products from lysates of cells expressing GST-fusion products of the Sec15p constructs described in A were analyzed by standard SDS-PAGE and western blotting with the indicated antibodies. The purified Sec15-GST constructs were resolved on an SDS-PAGE gel and the gel was analyzed by western blotting using anti-GST antibodies. Lysates were loaded to indicate that there were similar amounts of Sec10p present in all lysates although only the full-length Sec15p containing the extreme N-terminal region can pull-down Sec10p. (D) Diagram of the protein-protein interactions of Sec15.





Right arrow Return to article