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Fig. 7. Sec15-GFP recovers from photobleaching more slowly in the presence of latrunculin A. (A) Strain NY2445 containing Sec15-GFP (Sec15GFP) expressed from a fusion construct integrated as the sole copy of SEC15 at the SEC15 locus was grown overnight and used in photobleaching experiments as described in the Materials and Methods. Two representative recovery curves generated from photobleaching Sec15-GFP in the presence of DMSO alone or DMSO + 200 µM latrunculin A (Lat A) are shown. (B) In order to determine how many modes of action were causing Sec15-GFP recovery after photobleaching, the natural log of each point from the example recovery curve from part A was plotted against time. The straight line through the data indicates that only one mode of recovery was detectable for each. (C) The half-time of recovery for specimens incubated with Lat A was compared with the half-time of recovery for untreated specimens and found to be approximately four times longer; 90±16 seconds (n=13) versus 22±5 seconds (n=15).
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