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Figure 1


Fig. 1. Inhibition of adipogenesis in PKB{alpha}–/– MEF cells. (A) Two days after confluence, MEF cells were induced to differentiate into adipocytes with IBMX, dexamethasone and insulin for 48 hours followed by insulin alone every 2 days. Cells were stained with Oil-Red O at 8 days post-induction. (B) Expression of adipocyte-specific genes after stimulation of the adipose differentiation process in wild-type and PKB{alpha}–/– MEFs. Total RNA was extracted from cells at the times indicated and analysed by RT-PCR as described in the Materials and Methods.





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