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Figure 2


Fig. 2. Levels of the three PKB isoforms and the phosphorylation status of PKB in wild-type and PKB{alpha}–/– MEFs during adipose conversion. Total cellular extracts were prepared from cells before (day 0) or at 2, 5, 8 and/or 12 days after adipose induction treatment and analysed by western blotting with PKB{alpha}-, PKBß- and PKB{gamma}-specific antibodies (A) or with phospho-PKB specific antibodies (pSer473 and pThr308) (B). Blots were reprobed with anti-actin antibodies to control for equal loading.





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