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Figure 3


Fig. 3. Ectopic expression of PKB{alpha} by retrovirus infection restores adipogenesis in PKB{alpha}–/– MEFs. (A) PKB{alpha}–/– MEFs were infected with retrovirus expressing HA-PKB{alpha} or vector only. After puromycin selection, extracts of cells were analysed by western blotting for the expression of HA-PKB{alpha}. (B) Cells were exposed to the adipogenic cocktail and stained with Oil Red O after 15 days. (C) RT-PCR analysis of adipose markers PPAR{gamma} and aP2 in PKB{alpha}–/– MEFs infected with vector or HA-PKB{alpha}-expressing retrovirus and submitted to adipose differentiation.





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