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Figure 5


Fig. 5. PKB{alpha} induces Klf15 during adipose conversion of MEFs. (A) Microarray profile of Klf15 in wild-type and PKB{alpha}–/– MEFs during the first 2 days after hormonal stimulation. (B) Total RNA was extracted from cells at the times indicated and analysed by RT-PCR. (C) RT-PCR analysis of Klf15 gene expression after 24 hours of adipogenic treatment of PKB{alpha}–/– MEFs infected with vector or HA-PKB{alpha} expressing retrovirus. (D) PKB{alpha}–/– MEFs were infected with retrovirus expressing (m/p)-HA-PKB{alpha} or vector only. Following puromycin selection, lysates were analysed by western blotting for the expression of a constitutively active form of PKB{alpha}. (E) Total RNA was extracted from cells 3 days postconfluence and analysed by RT-PCR as described in the Materials and Methods.





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