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Figure 3


Fig. 3. hFBNRG3 is a surface-exposed plasma membrane protein. (A) Confocal microscopy images show plasma membrane location of the hFBNRG3 polypeptide (COS-7 cells were transfected with hFBNRG3-myc, incubated with MG-132 and processed for immunocytochemistry as described in Materials and Methods). (B) hFBNRG3 is also inserted to the plasma membrane of the neuronal-model cell line PC12. (C) CFP-hFBNRG3 polypeptide localizes on the plasma membrane of COS-7 cells. (D) CFP-SMDF, a plasma membrane neuregulin, was used as control. (E) The soluble CFP showed a wide distribution pattern. Arrows indicate plasma membranes. Bars, 10 µm. (F) hFBNRG3 is considerably associated with the insoluble fraction of crude plasma membrane preparations (P) but not with the soluble fraction (S). SMDF was used as a control. (G) Biotinylation increased the recovery of neuregulin in the streptavidin precipitates, showing that hFBNRG3 is a surface exposed polypeptide. Lysate of nonbiotinylated cells was used as control.





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