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Fig. 8. NRG3 is a survival factor for oligodendrocytes. (A) Serum-starved oligodendrocyte precursor cultures were immunostained with anti-NG2 antibody; a.1 and a.3 overlays; a.2 and a.4 Hoechst dye staining of nuclei. Apoptotic (arrows) and non-apoptotic (arrowheads) cells from 750 to 1500 NG2-positive cells per experiment were counted. Bars, 10 µm. (B) The results of three different experiments are shown. GST-NRG3 (150 nM) decreased apoptosis to similar levels found in non-serum-starved cultures. GST-purified protein was used as control. Data are given as mean ± s.e.m.; the Student's t-test was used (*P
0,015). (C) Recombinant neuregulin 3 increases the phosphoryaltion of Akt in a dose-dependent way in the rat C6 glioma cell line. Recombinant neuregulin 1 (GST-SMDF) was used as a control. The densitometric quantification of a typical experiment is shown. (D) Time course of Akt phosphorylation in oligodendrocyte precursor cultures. (E) Inhibition of PI 3-kinase pathway with 20 µM LY294002 abrogates the anti-apoptotic effect of recombinant NRG3. Data are given as mean ± s.e.m.; the Student's t-test was used, *P0.015.
