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Figure 8


Fig. 8. Interaction of AM and CGRP with endothelial EndoCL. Subcellular distribution of EndoCL in hDMVECs before treatment (Control) after exposure to AM (AM, 100 nM for 15 minutes) with or without antagonists (AM22-52 and CGRP8-37, 1 µM) was assessed by immunofluorescence. Cells were processed for immunofluorescence immediately after exposure. Immunofluorescence was performed using anti-hCL antibody LN-1436 and FITC-conjugated secondary antibody. DAPI was used to counterstain cell nuclei. Figures are representative merged images of two independent experiments. Cell surface (green arrows) and internalised (green arrowheads) receptor portions are indicated.





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