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Figure 6


Fig. 6. Enhancement of ubiquitylation of Dock180 in HEK293T cells re-plated on fibronectin-coated dishes. (A) HEK293T cells were transiently transfected with the indicated plasmids for Dock180 and HA-ubiquitin and re-plated on fibronectin-coated dishes for the indicated duration (top of the panel); lysates in the membrane fraction were subjected to in vivo ubiquitylation assay. Sus, suspended cells; FN, fibronectin. Ratio represents the relative signal intensity of Dock180 in the membrane fraction in lane 1 as 1.0 (bottom of the panel). (B) Inhibition of ubiquitylation of Dock180 by Elmo1. HEK293T cells were transiently transfected with the indicated plasmids, and the re-plating assay and in vivo ubiquitylation assay were performed as in A.





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