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Figure 6


Fig. 6. Expression of C6st1 mRNA in Schwann cells in 7 dpc nerves. Fluorescence in situ hybridisation for C6st1 mRNA in combination with fluorescence immunohistochemistry for either S100 (A-H) or GFAP (I-P). Images represent both transverse (A-D and I-L) and longitudinal (E-H and M-P) cryosections of 7 dpc sciatic nerves at the crush site. Phase-contrast views of the tissues are shown in the right-hand column. Merges of immunocytochemistry and hybridisation images are shown (C,G,K and O) with enlarged views (insets) of the boxed areas (C and K). Only some of the S100-positive Schwann cells expressed the C6st1 transcript (arrows) but all C6st1-expressing Schwann cells were GFAP-positive. Bars, 100 µm (A-P); 50 µm (insets).





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