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Figure 1


Fig. 1. Immunoblots demonstrating that VAMP7 is found in enterocytes and concentrated on PCTVs. (A) Immunoblot of rat intestinal ER (30 µg protein) separated by SDS-PAGE, transblotted to nitrocellulose membrane, and probed with anti-VAMP7 antibody. The molecular sizes of identified bands are indicated. (B) Immunoblot of ER membrane separated by 2D SDS-PAGE (8-18%). ER membranes (200 µg) were solubilized and separated first by pI and subsequently by molecular size (Materials and Methods). The proteins were transblotted onto nitrocellulose membranes and probed with anti-VAMP7 antibodies. Detection was by ECL. (C) Immunoblots of VAMP7 in different rat organs. of Post nuclear supernatant (30 µg protein) of rat intestinal (Inst.), rat kidney (Kid.), and rat liver (Liv.) were separated by 12% SDS-PAGE, transblotted onto nitrocellulose membranes and immunoblotted for VAMP7. Bands at 25 and 18 kDa are indicated.





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