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Figure 6


Fig. 6. Distribution of VAMP7 in the postnuclear supernatants of rat intestine, liver and kidney. Post-nuclear supernatant (2 mg protein) of intestine (A), liver (B) and kidney (C) were separated by 10-40% iodixanol gradient (Materials and Methods) and the gradient resolved by aspiration. Fractions (50 µl) were separated by 12% SDS-PAGE, transblotted to nitrocellulose membranes, and VAMP7 was identified by immunoblotting with anti-VAMP7 antibodies.





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