spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. PTEN and GFP-PTEN are enriched at microtubules in N1E-115 cells. (A) NIE-115 cells were labelled with anti-ßIII-tubulin (left) and anti-PTEN (middle) antibodies. Similarly to primary DRG neurons, there is an accumulation of endogenous PTEN with microtubules in N1E-115 cells. (B,C) NIE-115 cells were transfected with a control siRNA (B) or PTEN specific siRNA (C), and labelled with the anti-PTEN antibody (left) and Phalloidin to visualise F-actin. Confocal micrographs, taken with identical scanning parameters, demonstrate the loss of PTEN signal in the PTEN siRNA-treated cells. (D) Similarly to the distribution of endogenous PTEN, GFP-PTEN accumulates with microtubules in N1E-115 cells. Bars, 10 µm.





Right arrow Return to article