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Fig. 3. Human Sec31B generates multiple transcripts by alternative mRNA splicing. Three cDNA libraries were examined for alternate exon utilization by PCR. Representative PCR results are shown in the supplementary material Fig. S1. (A) Alternative splicing map of Sec31B representing experimentally observed patterns of exon utilization, based on PCR analysis and EST data available in GenBank. Constitutive exons (green) and exons alternatively utilized (red) are shown along with the frequency of their detection in the examined transcripts. Of the total of 29 exons identified, 16 exons were constitutively present in all transcripts. (B) Examples of four alternatively spliced transcripts depicting their exon utilization. Arrows represent the open reading frames with the predicted molecular mass of the protein products. Although an open reading frame downstream of the stop codon in exon 13 exists, no evidence was identified for the utilization of initiation sites other than that in exon 2. Notice that, if exon 4 is not utilized, then exon 5 contributes an out-of-frame stop codon. (C) Cartoon representing all patterns of exon utilization experimentally identified in this study. Exons encoding WD repeats and proline-rich sequences are indicated.
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