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Figure 2


Fig. 2. pHDV of mature trophozoite-stage D10' parasites as reported by BCECF (pKa ~7.0), Fluorescein (pKa ~6.4), Oregon Green 514 (pKa ~4.7) and CL-NERF (pKa ~3.8), all at a loading concentration of ~28 µM dextran. The three different pH calibration methods described in Materials and Methods each report a consistent linear relationship between dye pKa and pHDV (R2>0.99, P<0.05 in all cases), with the reported pHDV ranging from ~3.7 to ~6.5. The data represent the means (error bars show s.e.m.; where not shown, error bars fall within the symbol) of at least three independent experiments. The dotted line indicates data that would be obtained if pHDV were equal to dye pKa.





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