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Figure 4


Fig. 4. Comparison of pHDV in D10' parasites at the early trophozoite stage (16-22 hours post-invasion) and at the mature trophozoite stage (32-38 hours post-invasion) as determined using either ~40 µM Fluorescein (open symbols) or ~70 µM Oregon Green 514 (closed symbols) (i.e. ~28 µM dextran in the loading solution in each case), calibrated using the nigericin/high-K+ method. For both dyes, the reported pHDV changed significantly as the intraerythrocytic parasites developed from the early to the mature stage (P<0.05). The data represent the means (± s.e.m.) of at least three independent experiments, each derived from at least three replicate measurements per experiment.





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