spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. PML nuclear bodies increase in number during S phase. (A) Asynchronous human SK-N-SH cells were followed by DIC to determine relative cell cycle position after mitosis over an 18 hour period prior to immunolabelling for cyclin A. (B) Human U-2 OS cells stably expressing GFP-PML IV were synchronised at the G1-S border with aphidicolin and released into S phase for 2-6 hours. PML nuclear body number versus time is shown. (C) Asynchronous U-2 OS cells expressing diHcRed-PCNA (PCNA) and GFP-PML IV (PML) were observed for 2 hours by spinning-disk confocal microscopy. Images shown are compressed z-stacks. Newly replicated PML nuclear bodies are indicated by yellow arrowheads. (D) PML nuclear body doublets are present in SK-N-SH cells in mid S phase (arrowheads). Bars, 5 µm.





Right arrow Return to article