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Figure 3


Fig. 3. PML protein within MAPPs is not degraded by the 26S proteasome pathway during mitosis. (A) Mitotic HeLa cells were prepared by shake-off from an asynchronous population and plated on coverslips in medium containing 20 mM MG132 and allowed to grow for 2 hours (2H), 4 hours (4H) and 6 hours (6H). Cells were then fixed, processed for the immunodetection of PML (red), SC35 (green) and DNA was counterstained with DAPI. (B) The proteasome does not contribute to the loss of MAPPs in G1 nuclei. The percentage of cells containing PML nuclear bodies was similar between control (blue) and MG132 treated (purple) HeLa cells and no statistical difference was observed for the number of bodies per cell (data not shown). DNA was counterstained with DAPI (blue). Bar, 5 µm.





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