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Figure 2


Fig. 2. Regulation of CT-1 and HIF-1{alpha} expression in embryoid bodies by endogenous ROS. Embryoid bodies were incubated from day 2 to day 8 of cell culture with 20 µM vitamin E. On day 8 protein expression of CT-1 and HIF-1{alpha} was assessed by semiquantitative immunohistochemistry in whole-mount embryoid bodies. The images show representative embryoid bodies labeled with antibodies either against CT-1 or HIF-1{alpha} which remained untreated (a,c) or were treated with vitamin E (b,d). *P<0.05, significantly different to levels in the untreated control.





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