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Figure 3


Fig. 3. Upregulation of CT-1 and HIF-1{alpha} protein and mRNA upon treatment with prooxidants and hypoxia. (A,B) Embryoid bodies were treated on day 4 with menadione (20 µM) and H2O2 (10 µM). Protein (A) and mRNA (B) levels were examined 24 hours later. The images above A show representative embryoid bodies labeled with antibodies against CT-1 (left panel) and HIF-1{alpha} (right panel) which remained either untreated (a,d) or were treated with menadione (b,e) or H2O2 (c,f). (C,D) Embryoid bodies were treated on day 4 of cell culture with either physiological (1% O2) or chemical (CoCl2) hypoxia and protein (C) and mRNA (D) levels were examined 24 hours later. The images above C show representative embryoid bodies labeled with antibodies against CT-1 (left panel) and HIF-1{alpha} (right panel) which remained either untreated (a,d) or were treated with 1% O2 (b,e) or CoCl2 (50 µM) (c,f). *P<0.05, levels are significantly different to those in the untreated controls. Bars, 200 µm.





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