spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Upregulation of NADPH oxidase subunits upon incubation of embryoid bodies with menadione and CoCl2. (A) Protein expression of the NADPH oxidase subunits p22-phox, p47-phox, p69-phox, Nox-1 and Nox-4 which was determined 24 hours after treatment of 4-day-old embryoid bodies by semiquantitative immunohistochemistry. (B) mRNA expression of Nox1 and Nox4 upon either menadione (20 µM) or CoCl2 (50 µM) treatment. (C) The upregulation of CT-1 by menadione and CoCl2 was inhibited by preincubation with the NADPH oxidase inhibitors DPI (10 µM) or apocynin (10 µM). Embryoid bodies were treated at day 4 of cell culture with either menadione (20 µM) or CoCl2 (50 µM) in the presence of either DPI or apocynin. After 24 hours, CT-1 protein expression was determined by semiquantitative immunohistochemistry. The micrographs are representative embryoid bodies either untreated (a), or treated with menadione (b), CoCl2 (c), DPI (d), DPI + menadione (e), DPI + CoCl2 (f), apocynin (g), apocynin + menadione (h), apocynin + CoCl2 (i). *P<0.05, significantly different from levels in the control. #P<0.05, significantly different to levels in menadione- and CoCl2-treated samples before addition of inhibitors. Bar, 400 µm.





Right arrow Return to article