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Figure 4


Fig. 4. Varp interacts with Rab21 and Rab5a. Coimmunoprecipitation of the full-length Varp protein and Rab5a or Rab21, or their mutant forms. Myc-tagged Varp (Varp-Myc) and each of the GFP-tagged Rabs were coexpressed in HEK293T cells. Whole-cell lysates were immunoprecipitated with an anti-Myc antibody and precipitates were analyzed by western blotting with an anti-GFP antibody. Aliquots of whole-cell lysates were also examined by western blotting with anti-GFP or anti-Myc antibodies.





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