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Figure 5


Fig. 5. Distribution of Sp1 and Sp3 throughout the mitosis stages. MCF-7 cells were grown on coverslips in estrogen-complete medium, fixed, and double labeled with anti-Sp1 and anti-Sp3 antibodies. DNA was stained by DAPI. Sp1 and Sp3 distributions were visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merged images signifies colocalization. Bars, 5 µm.





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