spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. Mitotic colocalization of Sp1 with F-actin. MCF-7 cells were grown on coverslips in estrogen-complete medium, fixed, and labeled with rabbit polyclonal anti-Sp1 antibodies and mouse monoclonal anti-calnexin or anti-cytokeratin 8 or anti-cytokeratin 18 antibodies. F-actin was labeled by Alexa-Fluor-488-conjugated phalloidin. DNA was stained by DAPI. Spatial distribution was visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merge images signifies colocalization. Bar, 5 µm.





Right arrow Return to article