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Figure 1


Fig. 1. Protein components of melanosomes. (A,B) Early melanosomes and CCV isolated from M14 or MNT-1 cells were analyzed by IB with antibodies against Pmel17, AP1{alpha}, AP2{alpha} and TfR, as indicated. Experiments were performed twice with comparable results. (C) Pmel17 was immunoprecipitated using {alpha}PEP13h and was then immunoblotted with antibodies as noted. (D) Melanoma cells as noted were fixed and double stained for Pmel17 (red) and AP1{gamma} or AP2{alpha} (green) as indicated. Representative images for each combination are shown. Bars, 10 µm.





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