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Figure 2


Fig. 2. AP1 and AP2 colocalization with Pmel17. Melanoma cells were fixed, embedded in resin and double immunostained for Pmel17 with 25 nm gold (25) and AP1 or AP2 with 10 nm gold (10). Colocalization of Pmel17 (black arrows) with AP1 or AP2 (white arrows) was observed in CCV (A and D), stage I melanosomes (B and E) and stage II melanosomes (C and F). Note that AP2-vesicles with or without Pmel17 were observed in stage I melanosomes (E, inset) and inside vesicles in stage II melanosomes (F, inset). Bars, 0.1 µm.





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