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Figure 5


Fig. 5. Cytoplasmic sorting is restored by µ1B in SKMel-28 cells. (A) M14, MNT-1 and SKMel-28 cells were fixed and double stained for Pmel17 (red) and LDLR (green). Note the restricted distribution of Pmel17 and LDLR in SKMel-28 cells. (B) Z-scan analysis shows localization of LDLR with Pmel17 at the upper plasma membrane of MNT1 cells. Note, LDLR and Pmel17 were retained in the perinuclear area (arrows). (C) SKMel-28 cells were transfected with µ1A or µ1B and were cultured for 2 days. Cells were processed for staining of Pmel17 (red) and LDLR (green) by dual immunofluorescence and were analyzed by confocal microscopy. Note that transfection of µ1B restored cytoplasmic and plasma membrane localization (arrows) for both Pmel17 and LDLR. Representative images from each experiment are shown. Bars, 20 µm.





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