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Figure 2


Fig. 2. Kinetics of nuclear import of STAT2-EGFP. (A) LMB induces nuclear accumulation of STAT2-EGFP. NIH3T3 cells stably expressing STAT2-EGFP (top) or STAT1-EGFP (bottom) were treated with LMB (10 ng/ml). Subcellular distribution of STAT2-EGFP and STAT1-EGFP was examined before and at the indicated time points after LMB treatment by fluorescence microscopy. (B) NIH3T3 cells expressing STAT2-EGFP were treated for 2 hours with LMB (10 ng/ml) and subjected to selective FRAP. The indicated area in the nucleus was bleached for 40 seconds. Subsequently, recovery of unbleached STAT2-EGFP into the nucleus was monitored at low-power every 100 seconds. Fluorescence intensity is shown in false color code (intensity increases from blue to red). (C) NIH3T3 were treated as described in B; cells expressing STAT1-EGFP were subjected to selective FRAP.





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