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Figure 4


Fig. 4. Stimulation of IFN-ß blocks nuclear export of STAT2-EGFP. (A) U6A cells stably expressing STAT2-EGFP were treated with IFN-ß. At different times after treatment cytoplasmic FLIP analysis was performed using the same settings as described in Fig. 3. The fluorescence intensities of STAT2-EGFP in the nucleus of the bleached cells were measured, normalized to the total fluorescence of the unbleached cells and plotted over time. Relative fluorescence intensities at the indicated time periods after IFN-ß stimulation are shown. The localization of STAT2-EGFP during IFN-ß stimulation was documented by showing representative cells for the indicated time points. (B) 2fTGH cells expressing p50-GNES were subjected to cytoplasmic FLIP analysis. Relative nuclear fluorescence intensities are shown for untreated, IFN-ß-stimulated and LMB-treated cells. Insets show confocal images of representative cells. Colors of graphs reflect individual experiments.





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