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Figure 5


Fig. 5. STAT2 contains a strong constitutive nuclear export signal. (A) C243 cells were transfected with either STAT1-GNLS or STAT2-GNLS as indicated. Localization of the fluorescent proteins was determined in untreated cells by fluorescence microscopy. The effect of LMB was monitored in the same cells on STAT2-GNLS 15 and 30 minutes after treatment. (B) C243 cells transfected with STAT2-GNLS or STAT1-GNLS were subjected to cytoplasmic FLIP analysis using the same settings as described in Fig. 3A. The nuclear fluorescence intensities of the bleached cells were determined in different experiments, normalized to the total fluorescence of the unbleached cells and plotted over time. The relative nuclear fluorescence intensities of STAT2-GNLS (solid lines) and STAT1-GNLS (broken lines) are shown during a time period of 300 seconds. Colors of graphs reflect individual experiments.





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