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Figure 5


Fig. 5. Immunogold transmission-electron microscopy for BSF T. brucei. (A) Two vesicles labelled, one distinct MCA5 only (10 nm gold rabbit anti-sheep antibody) and one with RAB11 (6 nm gold goat anti-rabbit antibody) and MCA5. The particle groupings indicate an underlying bounding structure in the cytoplasm. (B) Presence of 10-nm and 6-nm gold particles at the periphery of a compartment. White arrowheads indicate bounding membrane. (C) Further example of RAB11 staining close to the flagellar pocket (D) Localisation of MCA5 only (white arrowhead), RAB11 with MCA5 (white arrow) and RAB11 only (black arrowhead) in T. brucei. Black arrow indicates surface coat; fp, flagellar pocket. Bars, 50 nm.





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