spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Recycling of VSG. Wild-type, {Delta}mca2/3{Delta}mca5 and triple RNAi lines, with or without induction with tetracycline for 8 hours. Parasites were biotinylated at 4°C prior to incubation at 37°C for 5 minutes in HMI-9 medium. Remaining surface label was then cleaved by incubation with 50 mM glutathione at 4°C. Cells were prepared for fluorescence microscopy both immediately after the glutathione incubation and following a 5- or 10-minute chase period in HMI-9 medium at 37°C. Parasites were stained with Texas-Red-conjugated streptavidin (red). DNA was visualised with DAPI (blue). Insets, DIC images of the parasite. Bars, 10 µm.





Right arrow Return to article