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Figure 4


Fig. 4. Overproduction of Fmi in the wild-type background caused underdevelopment of dendrites. (A) Dorsal clusters in a control embryo of 20-22 hours AEL where all da neurons expressed GFP. (B) Overproduction of the wild-type form of Fmi resulted in poor dendritic growth and a decrease in the number of branches. (C) Overproduction of {Delta}N::EYFP did not cause an obvious morphological defect. Genotypes were IG1-2 Gal109(2)80 UAS-GFP[S65T]/IG1-2 Gal109(2)80 UAS-GFP[S65T] (A), IG1-2 Gal109(2)80 UAS-GFP[S65T]/IG1-2 Gal109(2)80 UAS-GFP[S65T]; UAS-fmi/UAS-fmi (B), and IG1-2 Gal109(2)80 UAS-GFP[S65T]/IG1-2 Gal109(2)80 UAS-GFP[S65T]; UAS-{Delta}N::EYFP/UAS-{Delta}N::EYFP (C). Bar, 30 µm. Embryos that were homozygous for IG1-2 Gal109(2)80 UAS-GFP[S65T] did not hatch, so we could not observe how Fmi overexpression in this genotype that had total four copies of Gal4 affected dendrite morphogenesis at larval stages.





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