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Fig. 5. Fmi::EYFP prevented the dorsal-dendrite-overlap phenotype, whereas N::EYFP did not. (A-C and D-F) Dorsal front views of abdominal segments at 32-35 hours AEL and those at about 40 hours AEL, respectively. (A-C) A large subset of da neurons in dorsal clusters expressed GFP. (D-F) Essentially all da neurons except for ddaC were ablated in dorsal clusters of one hemi-segment and its contralateral counterpart by laser. Blue circles indicate cell bodies of the ddaC. Brackets indicate dendritic terminals that met midway between contralateral da neurons. Anterior is at the top left. (G-I) Tracing of dendritic branches that are shown in the respective panels D-F above. Dendrites that belonged to one of the adjacent hemi-segments are green or magenta, whereas intertwining branches that were difficult to track down are white. (A,D) Control larvae in which dendritic branches covered the whole body walls with minimum overlap. (B and E) In fmi mutant larvae, dendritic terminals that had extended from contralateral sides did not turn away, but rather overlapped or crossed with each other (bracket). Fmi::EYFP expression in da neurons in the fmi mutants rescued the larvae from the overlap phenotype (C), whereas N::EYFP expression did not confer an inhibitory interaction between terminals (F). Genotypes were Gal109(2)80 UAS-GFP[S65T]/IH1 UAS-GFP[S65T] (A and D), Gal109(2)80 UAS-GFP[S65T] fmi⁷²/IH1 UAS-GFP[S65T] fmi^E59 (B and E), and Gal109(2)80 UAS-GFP[S65T] fmi⁷²/IH1 UAS-GFP[S65T] fmi^E59; UAS-fmi/UAS-fmi (C), and Gal109(2)80 UAS-GFP[S65T] fmi⁷²/IH1 UAS-GFP[S65T]fmi^E59; UAS-N::EYFP/UAS-N::EYFP (F). Bars in I: 20 µm for A-C and 17 µm for D-I.

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