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Figure 7


Fig. 7. {Delta}N::EYFP expression decreased the level of Fmi at intercellular boundaries in wing epithelia. (A-I) {Delta}N::EYFP was expressed by ptc-Gal4 in wing imaginal discs of 3rd instar larvae. Anterior is to the left. Imaginal discs were stained for endogenous Fmi (A, D, G; magenta in C,F,I), EYFP (B,E,H; green in C,F,I). (C,F,I) Merged images. (D-F) Higher-magnification images of AP boundaries. (A-F) When adjacent cells strongly expressed {Delta}N::EYFP, the level of endogenous Fmi was reduced at cell-cell boundaries. (G-I) Boxed area in F shown higher-magnification image in I. Endogenous Fmi was localized at boundaries of cells that expressed {Delta}N::EYFP at distinct levels (arrowheads in G,H). Bar in I: 11.5 µm for A-C; 5.5 µm for D-F; 2 µm for G-I.





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