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Fig. 9. Effects of Fmi or 
N::EYFP overexpression on planar cell polarity. (A-M) The effect of N::EYFP expression on planar polarity was addressed in (A-D,I,J) adult wings, (E-H) a pupal wing at 30 hours after puparium formation, and (K-M) an adult notum. Anterior is at the top (A-M), and proximal is to the left (A-J). (A) Control wing. Inset shows higher-magnification image of the boxed area. (B-D) Either (B) full-length Fmi, (C) N::EYFP or (D) Fz were expressed in a region between vein 3 and vein 4 by using ptc-Gal4, which drives short-range gradient expression along the anterior-posterior axis of the wing. Arrowhead in D indicates probable peak of the expression level in. Arrows indicate directions of reoriented wing. (E-H) Triple-staining of a N::EYFP-expressing pupal wing for (E) EYFP, (F) endogenous Fmi (see magenta in H) and (G) Dsh (see green in H). A small area that straddled the boundary of the expression domain is shown at high magnification. In contrast to the normal preferential P-D distribution (arrow in H), endogenous Fmi was located substantially at A-P boundaries (arrowhead in H) in the 2-3 cells-wide stripe adjacent to the N::EYFP-producing cells. See text for details. Bar in E: 5 µm for E-H. (I-M) N::EYFP-expressing clones were identified by X-Gal staining. Yellow-boxed areas in I and K are shown magnified in J and L; magenta-boxed area in K is shown enlarged in M. Notice that wing hairs (black box in J) and trichomes (L,M) are pointing away from the clone boundaries. The relevant genotype was hs-FLP; AyGal4 UAS-lacZ/UAS-N::EYFP.
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