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Figure 10


Fig. 10. Cells lacking Cdc14p display impaired invasive and hyphal growth. (A) Cells of the indicated genotype were grown on YPD agar plates at 30°C for 7 days. Colonies were photographed before and after the plates had been washed. After washing, transverse sections (TS) were cut from the plates and photographed. Bar, 5 mm. (B) Colony morphology of the indicated strains grown on different hypha-inducing media after 5 days at 30°C. (C) Overnight cultures of strains JC190 (CDC14/CDC14) and JC12 (cdc14{Delta}/cdc14{Delta}) were diluted in pre-warmed YPD plus 5% serum at 37°C. At the indicated times, cells were removed and fixed to monitor response (% evagination) and the length of germ tubes. (D) Analysis of ECE1 and HWP1 expression by northern blot in CDC14/CDC14 and cdc14{Delta}/cdc14{Delta} strains at different times after cells had been transferred to YPD plus 5% FCS at 37°C.





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