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Figure 2


Fig. 2. Localization of Cdc14p during yeast growth. (A) Time-lapse fluorescence microscopy of unbudded yeast cells transformed with a plasmid containing the pACT1-CDC14-GFP construct (JJ22). Cells were mounted on glass slides containing 2% agar and differential interference contrast (DIC) (rows 1, 3 and 5) or GFP (rows 2, 4 and 6), and pictures were taken every 15 minutes. Note the presence of the GFP signal at the bud neck after the nucleus has migrated to the daughter cell (arrow). (B) Images of a single cell that was simultaneously stained with Calcofluor White (CW) and GFP are shown, and the overlap of both images (Merge).





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