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Figure 2


Fig. 2. Phenotypic analysis of the new genetic interactors of incenpP(EP)2340. (A-B) Normal eye and wing morphology in control (w1118) strain. (C-D) Representative examples of eye and wing phenotypes observed in trans-heterozygous combinations of EMS-induced mutations over incenpP(EP)2340: (C) Reduced eyes in trans-heterozygous EC3959/incenpP(EP)2340. (D) Irregular nicked wing-shape in trans-heterozygous EC2519/incenpP(EP)2340 fly. (E,F) Defects in the primary spermatocytes in trans-heterozygous males as a result of abnormalities in the gonial mitoses. (E) Variation in the nuclear size in EC2519/incenpP(EP)2340 is an indication of defective chromokinesis. Arrows point to micronuclei, arrowhead to polyploid nucleus. (F) The presence of giant primary spermatocytes (arrows) in EC2394/incenpP(EP)2340 could be indicative of a defect in cytokinesis in gonial mitosis. (G) Wild-type primary spermatocyte cysts. (H,I) Abnormal (H) meiotic spindles and (I) tetrapolar spindles (arrows) in EC2394/incenpP(EP)2340. (J) Wild-type telophase I spindles. Dotted lines in H-J delimit the meiotic cyst. Bar, 10 µm.





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