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Fig. 5. Mitochondrial dysfunction in midA-/- cells. Mitochondria were stained with Mito Tracker Red (A) or Mito Tracker Green (B) and fluorimetric quantification of the respective fluorescence from similar numbers of wild type and mutant cells was carried out. (C) The amount of ATP was measured in a bioluminescence assay. (D) Cellular oxygen consumption from wild-type and midA- cells was measured in a Clark-type electrode. Significance of differences is indicated by the P value shown inside the panels; n.s., nonsignificant differences.