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Figure 1


Fig. 1. 2D maps of RSPs in 20S radial spoke fractions. Newly identified proteins whose sequences were completely determined are labeled in red. RSPs 15 and 18, marked in green, yielded peptides that matched regions of the genome where no gene model currently exists. Proteins identified previously are in blue. Those that remain to be identified are in black. RSPs 20, 22 and 23, which also were identified previously, are not visible because they are poorly stained by the non-formaldehyde silver stain (RSPs 20 and 23) or are too small to be retained in the gel (RSP22). The proteins were resolved in 12% (A) and 6% (B) gels following non-equilibrium isoelectric focusing. Figures only show the relevant area of pH 4.6-7.3 (A) and 5-6.5 (B). The acidic end is to the right. Horizontal bars indicate molecular markers of 130, 100, 83, 54, 40 and 24 kDa, from top to bottom.





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