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Fig. 2. Eggs laid by Cad99C mutant flies are permeable to the dye Neutral Red. (A-E) Eggs derived from (A) heterozygous Cad99C57A/+ or (B-E) homozygous mutant Cad99C57A/57A flies were dechorionated and incubated in the dye Neutral Red. Eggs derived from homozygous mutant flies showed a range of Neutral Red stainings from (B) undetectable to (C) uniform weak, (D) partially strong and (E) uniformly strong. (F) Quantification of the percentage of eggs with no, weak, partial and strong Neutral Red staining. The genotypes of the flies from which the eggs were derived are indicated. GE21034 is the parental fly line used to generate the Cad99C57A and Cad99C248A mutants. At least 750 eggs per genotype were analyzed.