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Figure 3


Fig. 3. Peroxisome formation in S. cerevisiae. The top two rows show a time course of peroxisome formation induced by expression of Pex3-YFP (green) (based on Hoepfner et al., 2005); the ER is visualized by constitutive expression of Sec63-CFP (red); blue false colour shows the phase contrast. Schematics below display the events that are taking place. At the onset of the induction experiment, no Pex3-YFP signal is present (0). 60 minutes later, the first detectable weak Pex3-YFP signal localizes to structures containing the perinuclear ER marker Sec63-CFP (60). At 90 minutes after induction, Pex3-YFP starts to concentrate into dots, which are frequently localized at the periphery of the ER (90). At 120 minutes after induction, the Pex3-YFP is localized exclusively to dot-like structures that are significantly brighter and no longer overlap with the Sec63-CFP signal (120). Finally, 5-10 individual Pex3-YFP dots per cell are discernible and show no apparent ER colocalization but instead mostly localize to the cell cortex (300).





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