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Figure 3


Fig. 3. Immunolocalization of palladin isoforms in cultured astrocytes. The myc-tagged, 90 kDa isoform of palladin was transfected into embryonic rat astrocytes with adenovirus. (D-F) Expressed 90 kDa palladin was detected 12 hours later with a monoclonal antibody directed against the N-terminal myc-epitope tag. (A-C) The endogenous 140 kDa isoform of palladin was detected with the polyclonal antibody a-4IgNT. The two isoforms strongly colocalize. Both isoforms are detected periodically along stress fibers (B and E) and both localize to cell-cell junctions (C and F). Bars, 50 µm.





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